| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 31 | Mixta calida | TGGCACCGACAATTCGCA | AGCCGAACTGTTGCAGCA | 60.28 | 60.20 | 195 | 100 |
100.00%
|
100.00%
|
| Primer 32 | Mixta calida | TATGCGTCAGGCTGTCCAC | TGCGCTTCGGCTGATACT | 59.78 | 59.11 | 286 | 100 |
100.00%
|
100.00%
|
| Primer 33 | Mixta calida | TGTATGCGTCAGGCTGTCC | TGCGCTTCGGCTGATACT | 59.78 | 59.11 | 288 | 100 |
100.00%
|
100.00%
|
| Primer 34 | Mixta calida | CCTGTATGCGTCAGGCTGT | TGCGCTTCGGCTGATACT | 59.78 | 59.11 | 290 | 100 |
100.00%
|
100.00%
|
| Primer 35 | Mixta calida | CCACTGCTATCAGCCTGCA | TGCGCTTCGGCTGATACT | 59.78 | 59.11 | 158 | 100 |
100.00%
|
100.00%
|
| Primer 36 | Mixta calida | AAGATCTGCGCTGGCCAA | ACGCTTGCGGTAATGGCT | 59.65 | 60.05 | 173 | 97 |
100.00%
|
100.00%
|
| Primer 37 | Mixta calida | AAAACGCCATCCGCTCCA | TGACGCACCTGCTCAAGAC | 59.97 | 60.30 | 244 | 97 |
100.00%
|
100.00%
|
| Primer 38 | Mixta calida | AGGGTAAAGGCGTGTGCT | AGCAGCGCTTTCAGCTCA | 58.84 | 59.97 | 160 | 97 |
100.00%
|
100.00%
|
| Primer 39 | Mixta calida | AAGGGTAAAGGCGTGTGCT | AGCAGCGCTTTCAGCTCA | 59.55 | 59.97 | 161 | 97 |
100.00%
|
100.00%
|
| Primer 40 | Mixta calida | TCAGCGTGAGGAAGATGGC | AACGCTTTGCCCTGTTGC | 59.78 | 59.58 | 243 | 97 |
100.00%
|
100.00%
|